P 398 Title: Experimental retinal toxicity of intravitreal injection of liposome encapsulated 5-fluorouridine

Eurppse : To evaluate the presence of Neuron Specific Enolase (NSE) in subretinal fluid (SRF? as a uotential marker for retinal damaee and to correlate these b&Vets ‘tith r&al detachment (RD) pzmmwem kS.E is a reliablemarkerofneuronallysisintbecerebro-~fluidinbraindamage. Levels of NSE in the aqueous humour are also elevated in retiwblastoma NSEhaskdetectedinalllayenofnmmal humanretinaexcepth4uller cells and retinal pigment epithelimn. Methods : SRF and blood samples were collected during RD before cryc4hempy (CT) in 3 cases (Group l), a&r CT in 18 cases (Group 2). be&Fmzd after CT in 2 cases (Croup 3). NSE was measured with a mtoassay (sensitivity In&l). We had no &zence values for subretinat fluid Normal range is c 15ng/ml. in aqueous humor (collected in 10patientspriortocatamctsmgery). ~:MeanlevelsofNSEinSRFingroup2(1264f881n%ml)significantly higher (p< 0.01) than those in group 1 (157 f 114 n&l). A lOfddin-wasnotioed~~intheSRFofbothpetientsofgroup 3. No conelation could be found between tbe levels of NSE and the duration of RD, it’s size, the presence of Pa visual recovery, visual field testing or .$e age of the pa!knt, with a 6 months follow up. L&&w!u : Elevatimt of NSE levels prim to crycUterapy suggest a modemte retinal lysis secondary to RD. Much higher levels of NSE found at?erCTintheSRFofthesepaUah suggestanimpcrtantr&aldamage relatedwiththisCT.NSEevaluationcouldbeusefultocom~retinal damage induced by diode laser therapy versus CT.